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M9480819.TXT
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1994-09-05
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Document 0819
DOCN M9480819
TI Quantitative determination of HIV-1 p24 antigen in HIV antibody positive
sera following alkaline dissociation of immune complexes.
DT 9410
AU Swack N; Ramirez M; Berberich S; Stapleton J; Hygienic Laboratory, Univ.
of Iowa, Iowa City.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:624 (abstract no. V-37). Unique
Identifier : AIDSLINE ASM94/94313126
AB Assays for the detection of HIV-1 antigen have been used for the
detection of circulating virus early in infection and also for
assessment of disease progression and response to antiviral therapy.
However HIV-1 p24 antigen may be complexed with HIV antibody and thus
escape detection by antigen capture immunoassay. Previous studies to
assay virus after acid dissociation of the immune complexes was found to
be time consuming and required large quantities of serum. These problems
were significantly reduced by the use of alkaline dissociation which was
significantly less labor intensive and required smaller amounts of
serum. Ninety seven serum specimens obtained from patients at the
University of Iowa AIDS Clinic were studied. All sera were found to be
HIV-1 antibody positive by Western Blot analysis. The semiquantitative
detection of p24 antigen was studied by antigen capture assays obtained
from commercial sources (Abbott Laboratories and Organon Teknika).
Antigen dissociation was performed with the Vironostika HIV-1 Antigen
Base Dissociation Assay. Both antigen assays without dissociation found
35% of the sera to be positive for the presence of p24 antigen.
Following treatment with the alkaline dissociation reagents 67% of the
same sera were positive for the presence of p24 antigen. All of these
could be neutralized by specific antibody to HIV. Quantitative p24
determinations performed by both the undissociation and alkaline
dissociation procedures were compared with patient lymphocyte
immunophenotyping studies.
DE Acquired Immunodeficiency Syndrome/*BLOOD/IMMUNOLOGY Antigen-Antibody
Complex/*BLOOD/CHEMISTRY/ISOLATION & PURIF Blotting, Western/METHODS
Comparative Study Human HIV Antibodies/*BLOOD HIV Antigens/BLOOD HIV
Core Protein p24/*BLOOD HIV-1/IMMUNOLOGY/*ISOLATION & PURIF
Immunophenotyping/METHODS Lymphocytes/IMMUNOLOGY Reagent Kits,
Diagnostic MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).